Difference between revisions of "Part:BBa K310000:Design"

 
(→‎Design Notes)
 
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<partinfo>BBa_K310000 short</partinfo>
 
<partinfo>BBa_K310000 short</partinfo>
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The biobrick prefix is attached just upstream of the sRNA.  This leads to the addition of 8 nucleotides (scar coding) to the 5' end of the sRNA, TACTAGAG, when combined with an upstream part (ie-promoter).  All sRNA's in E. coli carry an intrinsic terminator.  The suffix is located ~20 bp downstream of this terminator.
 
The biobrick prefix is attached just upstream of the sRNA.  This leads to the addition of 8 nucleotides (scar coding) to the 5' end of the sRNA, TACTAGAG, when combined with an upstream part (ie-promoter).  All sRNA's in E. coli carry an intrinsic terminator.  The suffix is located ~20 bp downstream of this terminator.
  
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An EcoRI site has been changed to a Gatttc site.  K310000 is now RFC10 compatible.
  
 
===Source===
 
===Source===

Latest revision as of 12:24, 6 November 2010

micA (sRNA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick prefix is attached just upstream of the sRNA. This leads to the addition of 8 nucleotides (scar coding) to the 5' end of the sRNA, TACTAGAG, when combined with an upstream part (ie-promoter). All sRNA's in E. coli carry an intrinsic terminator. The suffix is located ~20 bp downstream of this terminator.

An EcoRI site has been changed to a Gatttc site. K310000 is now RFC10 compatible.

Source

Escherichia coli MG1655

References