Difference between revisions of "Part:BBa K310017"
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<partinfo>BBa_K310017 short</partinfo> | <partinfo>BBa_K310017 short</partinfo> | ||
| − | + | [[Image:3_sRNA.png|thumb|Targeted Regulation by sRNAs:micF, micA, and gadY. sRNAs expressed under lac promoter.]][[Image:GadY_vs_lock_1.png|thumb|Comparison of target gene activation between gadY/gadX and key1/lock1 (J01008/J01010). gadY and key1 expressed under lac promoter]] | |
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| + | This part is the 3'UTR of the gadX mRNA. It contains the target sequence for gadY sRNA. Added to a protein coding sequence, the gadX 3'UTR will destabalize the mRNA transcript resulting in low expression. Upon the expression of gadY sRNA, the sRNA-mRNA interaction will stabalize the transcript resulting in activated gene expression. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | Use this part in conjunction to gadY sRNA (BBa_K310001). See gadY sRNA page. | ||
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Latest revision as of 12:07, 6 November 2010
gadX 3'UTR (gadY target)
This part is the 3'UTR of the gadX mRNA. It contains the target sequence for gadY sRNA. Added to a protein coding sequence, the gadX 3'UTR will destabalize the mRNA transcript resulting in low expression. Upon the expression of gadY sRNA, the sRNA-mRNA interaction will stabalize the transcript resulting in activated gene expression.
Usage and Biology
Use this part in conjunction to gadY sRNA (BBa_K310001). See gadY sRNA page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]