Difference between revisions of "Part:BBa K310000"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K310000 short</partinfo>
 
<partinfo>BBa_K310000 short</partinfo>
  
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micA is a small non-coding RNA regulator that binds to the 5' UTR of OmpA via non-perfect base pairing. The primary action of micA is the translational repression of OmpA by ribosome occlusion of OmpA mRNA and subsequent destabalization of the transcript. micA binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex.
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[[Image:MicF_Biobrick_mFOLD_small.png|thumb|mFOLD for this sRNA]][[Image:3_sRNA.png|thumb|Targeted Regulation by sRNAs:micF, micA, and gadY.  sRNAs expressed under lac promoter.]]
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micA is a small non-coding RNA regulator that binds to the 5' UTR of ompA mRNA transcript via non-perfect base pairing. The primary action of micA is the translational repression of ompA by ribosome occlusion of ompA mRNA and subsequent destabalization of the transcript. micA binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex, and specifies cellular localization of sRNA-mRNA interactions.
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'''note'''
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8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding).
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overexpression of hfq may affect efficacy of sRNA regulation, but pleiotropic effects observed in cell growth.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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micA sRNA is a post-transcriptional regulator.  Using in conjunction with transcriptional regulators yield clear and strong bistability of target gene expression (data not shown).  Endogenous to E. coli, micA regulates the expression of the outer membrane proteins in response to environmental stress conditions.
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Revision as of 08:02, 6 November 2010

micA (sRNA)

mFOLD for this sRNA
Targeted Regulation by sRNAs:micF, micA, and gadY. sRNAs expressed under lac promoter.


micA is a small non-coding RNA regulator that binds to the 5' UTR of ompA mRNA transcript via non-perfect base pairing. The primary action of micA is the translational repression of ompA by ribosome occlusion of ompA mRNA and subsequent destabalization of the transcript. micA binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex, and specifies cellular localization of sRNA-mRNA interactions.

note 8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding). overexpression of hfq may affect efficacy of sRNA regulation, but pleiotropic effects observed in cell growth.

Usage and Biology

micA sRNA is a post-transcriptional regulator. Using in conjunction with transcriptional regulators yield clear and strong bistability of target gene expression (data not shown). Endogenous to E. coli, micA regulates the expression of the outer membrane proteins in response to environmental stress conditions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]