Difference between revisions of "Part:BBa K310002"
Line 8: Line 8:
 
8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding)
 
8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding)
  
−
 
+
[[Image:3_sRNA.png‎|thumb|Targeted Regulation of GFP by sRNA micF, micA, and gadY.  sRNAs expressed under lac promoter]]
−
 
+
  
  

Revision as of 07:44, 6 November 2010

MicF (sRNA)

mFOLD for this sRNA

micF is a small non-coding RNA regulator that binds to the 5' UTR of ompF mRNA transcript via non-perfect base pairing. The primary action of micF is the translational repression of ompF by ribosome occlusion of ompF mRNA and subsequent destabalization of the transcript. micF binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex, and specifies cellular localization of sRNA-mRNA interactions.

note 8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding)

Targeted Regulation of GFP by sRNA micF, micA, and gadY. sRNAs expressed under lac promoter




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]