Difference between revisions of "Part:BBa K342001:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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===Applications of BBa_K342001===
 
===Applications of BBa_K342001===
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<h2>Results</h2>
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<p><i>E. coli</i> bacteria had been transformed with the pILI1 plasmid, which contains the <i>phaCAB</i> operon. This recombinant bacteria had been spread on LB agar plates, with or without 7% of glucose and nile red dye. As you can see on the photos below, the bacteria appear pink colored, directly on plates supplemented with glucose (on the left photo). However, the control without glucose doesn't reveal a pink coloration (on the right photo).</p><br/>
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<img src="http://lh4.ggpht.com/_Uc3bmii-yi0/TMf__pnKyLI/AAAAAAAAAl4/KBXck8LZerk/P1050468%20%282%29.JPG" />
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<p style="font-size:0.9em; text-indent:0px; text-align:center;"><em>LB agar plate with pILI1, with or without glucose 7% and nile red dye.</em><br/></p>
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<p>With a liquid culture on LB complemented with glucose (7%) and nile red dye, we observed the fluorescence by microscopy.</p><br/>
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<img src="http://lh6.ggpht.com/_Uc3bmii-yi0/TMgFqr4biTI/AAAAAAAAAmQ/Lp_33O0Td8c/Granule1.jpg" />
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<img src="http://lh5.ggpht.com/_Uc3bmii-yi0/TMgEslmyE_I/AAAAAAAAAmE/ODeTOjHAb4g/Granule2.jpg" /> <br/>
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<p style="font-size:0.9em; text-indent:0px; text-align:center;"><em>Observation of fluorescent granules by microscopy. </em><br/></p>
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===User Reviews===
 
===User Reviews===

Latest revision as of 19:47, 5 November 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K342001

Results

E. coli bacteria had been transformed with the pILI1 plasmid, which contains the phaCAB operon. This recombinant bacteria had been spread on LB agar plates, with or without 7% of glucose and nile red dye. As you can see on the photos below, the bacteria appear pink colored, directly on plates supplemented with glucose (on the left photo). However, the control without glucose doesn't reveal a pink coloration (on the right photo).



LB agar plate with pILI1, with or without glucose 7% and nile red dye.


With a liquid culture on LB complemented with glucose (7%) and nile red dye, we observed the fluorescence by microscopy.



Observation of fluorescent granules by microscopy.


User Reviews

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