Difference between revisions of "Part:BBa K382003"
Line 5: Line 5:
  
 
We used this vector for integration of several gene constructs into ''Arabidopsis''.[http://2010.igem.org/Team:Harvard/results]
 
We used this vector for integration of several gene constructs into ''Arabidopsis''.[http://2010.igem.org/Team:Harvard/results]
 +
 +
* LTP knock-down amiRNA and ihpRNA ([[Part:BBa_K382064|BBa_K382064]], [[Part:BBa_K382070|BBa_K382070]])
 +
* Ger3 knock-down ihpRNA ([[Part:BBa_K382066|BBa_K382066]])
 +
* BetVI knock-down ihpRNA ([[Part:BBa_K382068|BBa_K382068]])
 +
* LUT2 knock-down amiRNA ([[Part:BBa_K382058|BBa_K382058]])
 +
* LYC knock-down amiRNA ([[Part:BBa_K382060|BBa_K382060]])
 +
* Beta Ohase I knock-down amiRNA ([[Part:BBa_K382059|BBa_K382059]])
  
 
Plant transformed with this vector:
 
Plant transformed with this vector:

Revision as of 17:45, 5 November 2010

pORE Expression Series Vector with BioBrick MCS (Kan reistance)

Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has the constitutive promoter pENTCUP2 upstream of multiple cloning site for easy expression of genes in plants. Conforms to Silver BioBrick Assembly Standard 21.

We used this vector for integration of several gene constructs into Arabidopsis.[http://2010.igem.org/Team:Harvard/results]

Plant transformed with this vector:
BBa K382003 plant.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5269
    Illegal XbaI site found at 5284
    Illegal SpeI site found at 5298
    Illegal PstI site found at 5312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5269
    Illegal SpeI site found at 5298
    Illegal PstI site found at 5312
    Illegal NotI site found at 5275
    Illegal NotI site found at 5305
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5269
    Illegal BglII site found at 5182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5269
    Illegal suffix found in sequence at 5298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5269
    Illegal XbaI site found at 5284
    Illegal SpeI site found at 5298
    Illegal PstI site found at 5312
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 2181
    Illegal NgoMIV site found at 2772
    Illegal NgoMIV site found at 7160
    Illegal NgoMIV site found at 7795
    Illegal AgeI site found at 341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 7644
    Illegal SapI.rc site found at 7854