Difference between revisions of "Part:BBa K382021"
Line 5: Line 5:
  
 
We characterized[http://2010.igem.org/Team:Harvard/flavor/results] expression of the YFP-tagged version of this part in ''E. coli''. [[Part:BBa_K382040|BBa_K382040]]
 
We characterized[http://2010.igem.org/Team:Harvard/flavor/results] expression of the YFP-tagged version of this part in ''E. coli''. [[Part:BBa_K382040|BBa_K382040]]
 +
 +
2xYFP tags were attached to either N- (panel A) or C- terminus (panel B) of miraculin to ensure that folding was not hindered. In all cases relative YFP fluorescence had appreciably increased after 120 minutes as compared to the non-induced E. Coli.
  
 
YFP tag performance:
 
YFP tag performance:
 
<br>
 
<br>
 
[[Image:Mir_BrazzYFP_Fig_1-crop.jpeg|600px]]
 
[[Image:Mir_BrazzYFP_Fig_1-crop.jpeg|600px]]
 +
 +
Conclusion: We have confirmed that the BioBricked miraculin protein can be tagged with YFP and expressed from am IPTG inducible BioBrick promoter in E. coli. The flavor inverting function of miraculin expressed from E. coli has not been teasted, due to safety restrictions. Continued work will allow us to produce Miraculin in form that is safe for consumption.
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:58, 5 November 2010

Arabidopsis optimized Miraculin

Miraculin is a 'flavor inverting' protein, found naturally in the fruit of the plant fruit of Synsepalum dulcificum. Not sweet by itself, miraculin binds to taste receptors on the tongue, possibly altering the structure of the receptors and causing traditionally 'sour' flavors to be received as 'sweet'.

We characterized[http://2010.igem.org/Team:Harvard/flavor/results] expression of the YFP-tagged version of this part in E. coli. BBa_K382040

2xYFP tags were attached to either N- (panel A) or C- terminus (panel B) of miraculin to ensure that folding was not hindered. In all cases relative YFP fluorescence had appreciably increased after 120 minutes as compared to the non-induced E. Coli.

YFP tag performance:
Mir BrazzYFP Fig 1-crop.jpeg

Conclusion: We have confirmed that the BioBricked miraculin protein can be tagged with YFP and expressed from am IPTG inducible BioBrick promoter in E. coli. The flavor inverting function of miraculin expressed from E. coli has not been teasted, due to safety restrictions. Continued work will allow us to produce Miraculin in form that is safe for consumption.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]