Difference between revisions of "Part:BBa K371030"
 
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<partinfo>BBa_K371030 parameters</partinfo>
 
<partinfo>BBa_K371030 parameters</partinfo>
 
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[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(1).jpg]]
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[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(1).jpg]]  
 
[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(2).jpg]]
 
[[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(2).jpg]]
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Localization of P1-64 fusion with GFP(BBa_E0043) in E.coil. These pictures are take by confocal laser scanning microscope. Pdu BMC is indicated by arrow.

Latest revision as of 07:54, 5 November 2010

MPF(meta-prefix)+[pduP64+10*GS+GFP] fusion protein+MSF(meta-suffix)

This parts is a fusion protein between the N terminus 64 amino acid of pdu P protein and GFP protein(BBa_E0043). At each end is special DNA sequence that make this parts compatible to BBF RFC 53. The MPF and MSF is the abbreviation of meta-prefix and meta-suffix seperately. Because the N terminus is said to target pduP inside the pdu BMC, this part can be used to direct pduP-GFP chimera into BMC lumun.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal SapI site found at 3


USTC2010 Result of Fusion Protein P L-GFP(1).jpg USTC2010 Result of Fusion Protein P L-GFP(2).jpg Localization of P1-64 fusion with GFP(BBa_E0043) in E.coil. These pictures are take by confocal laser scanning microscope. Pdu BMC is indicated by arrow.