Difference between revisions of "Part:BBa K329035"
 
Line 2: Line 2:
 
<partinfo>BBa_K329035 short</partinfo>
 
<partinfo>BBa_K329035 short</partinfo>
  
−
<!-- Add more about the biology of this part here
+
This part carry the both enzyme Int and Xis of the conjugative transposon Tn916 under the control of the inducible promoter Pbad/AraC. The Tn916 is a "cut and paste" transposon which naturally move a 18kb DNA fragment carrying Tetracycline resistance. Tn916 is able to make intra and intercellular transfer thanks to a closed circular intermediate.
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 03:50, 5 November 2010

pBAD/araC - RBS - Xis_Int Tn916

This part carry the both enzyme Int and Xis of the conjugative transposon Tn916 under the control of the inducible promoter Pbad/AraC. The Tn916 is a "cut and paste" transposon which naturally move a 18kb DNA fragment carrying Tetracycline resistance. Tn916 is able to make intra and intercellular transfer thanks to a closed circular intermediate.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961