Difference between revisions of "Part:BBa K353002:Experience"
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===Applications of BBa_K353002=== | ===Applications of BBa_K353002=== | ||
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| + | Below is a graph of our flow cytometry data for part K353002 plotting GFP output for BW27783 cells: | ||
[[Image:Realdata.jpg]] | [[Image:Realdata.jpg]] | ||
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| + | Both of these graphs plot the output of part K353002 in BW27783 cells induced with a series of concentrations of L-arabinose, measured in molarity. The first is a histogram showing the distribution of expression levels at each concentration of L-arabinose. The second is the same data displayed as a cumulative distribution so that population medians are more apparent. | ||
[[Image:740px-Flow data.jpg]] | [[Image:740px-Flow data.jpg]] | ||
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| + | We measured a dose-response-curve of median output vs L-arabinose concentration in triplicate. This curve constitutes the basic characterization of part K353002, including measurement of the concentration of L-arabinose which gives half-maximal output, the steepness of transition from off to on, and the parts dynamic range. Fitting a Hill curve provided the following values plus or minus 95% confidence intervals: | ||
| + | Half-max induction: 143 +- 36 uM | ||
| + | Hill coefficient: 1.4 +- .6 | ||
| + | Fold Induction: 860 | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 00:50, 5 November 2010
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K353002
Below is a graph of our flow cytometry data for part K353002 plotting GFP output for BW27783 cells:
Both of these graphs plot the output of part K353002 in BW27783 cells induced with a series of concentrations of L-arabinose, measured in molarity. The first is a histogram showing the distribution of expression levels at each concentration of L-arabinose. The second is the same data displayed as a cumulative distribution so that population medians are more apparent.
We measured a dose-response-curve of median output vs L-arabinose concentration in triplicate. This curve constitutes the basic characterization of part K353002, including measurement of the concentration of L-arabinose which gives half-maximal output, the steepness of transition from off to on, and the parts dynamic range. Fitting a Hill curve provided the following values plus or minus 95% confidence intervals: Half-max induction: 143 +- 36 uM Hill coefficient: 1.4 +- .6 Fold Induction: 860
User Reviews
UNIQ9e947eca96fc2c43-partinfo-00000000-QINU UNIQ9e947eca96fc2c43-partinfo-00000001-QINU