Difference between revisions of "Part:BBa K313000"
(→‎Verification Experiment)
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=== Verification Experiment ===
 
=== Verification Experiment ===
  
−
Plasmid containing this part was transformed to Rosetta (DE3) plysS.
+
Plasmid containing this part was transformed to Rosetta (DE3) plysS strain which have T7 polymerase under the control of Lac promoter.
 
When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM
 
When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM
 
Fluorescence was measured and the results are shown below:
 
Fluorescence was measured and the results are shown below:

Revision as of 13:14, 2 November 2010

gfp generator with T7 promoter

gfp generator with T7 promoter

Usage and Biology

T7 RNA polymerase induces the expression of gfp from this part.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Verification Experiment

Plasmid containing this part was transformed to Rosetta (DE3) plysS strain which have T7 polymerase under the control of Lac promoter. When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM Fluorescence was measured and the results are shown below:

Graph 5.png


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719