Difference between revisions of "Part:BBa K339003:Design"
 
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===Design Notes===
 
===Design Notes===
  
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Obtained in plasmid form from the Betton labs in France (Betton et al., 2002).   Used PCR to amplify the gene out of the plasmid and add the biobrick prefix and suffix.
+
Obtained in plasmid form from the Betton labs in France (Betton et al., 2002). Used PCR to amplify the gene out of the plasmid and add the biobrick prefix and suffix.
  
  

Latest revision as of 00:32, 31 October 2010

malE31ΔSS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
    Illegal BamHI site found at 96
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58


Design Notes

Obtained in plasmid form from the Betton labs in France (Betton et al., 2002). Used PCR to amplify the gene out of the plasmid and add the biobrick prefix and suffix.


Source

etton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. Research in Microbiology. (153): 399-404.


References