Difference between revisions of "Part:BBa K339001:Design"
Emily Hicks (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K339000 short</partinfo> <partinfo>BBa_K339000 SequenceAndFeatures</partinfo> ===Design Notes=== ===Source=== Plasmid DNA was obtained from Jean-Betton labs ...) |
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| − | Plasmid DNA was obtained from Jean-Betton labs in France (Betton et al., 2002). | + | Plasmid DNA was obtained from Jean-Betton labs in France, amplified out of the plasmid through PCR and then subsequently biobricked (Betton et al., 2002). |
===References=== | ===References=== | ||
Latest revision as of 00:31, 31 October 2010
Mutant Maltose Binding Protein (malE31)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 435
Illegal BamHI site found at 171 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 133
Design Notes
Source
Plasmid DNA was obtained from Jean-Betton labs in France, amplified out of the plasmid through PCR and then subsequently biobricked (Betton et al., 2002).
References
Betton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. Research in Microbiology. (153): 399-404.
Kapust, R. and Waugh, D. (1999). Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Science. 8(8):1688-1674.
ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.