Difference between revisions of "Part:BBa K339002:Design"

 
Line 12: Line 12:
 
===Source===
 
===Source===
  
Part was obtained in plasmid form from Betton labs in France and then biobricked (Betton et al., 2002).
+
Part was obtained in plasmid form from Betton labs in France and then amplified out of the plasmid using PCR and subsequently biobricked (Betton et al., 2002).
  
 
===References===
 
===References===
  
 
Betton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. ''Research in Microbiology''. (153): 399-404.
 
Betton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. ''Research in Microbiology''. (153): 399-404.

Latest revision as of 00:08, 31 October 2010

malEΔSS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58


Design Notes

None


Source

Part was obtained in plasmid form from Betton labs in France and then amplified out of the plasmid using PCR and subsequently biobricked (Betton et al., 2002).

References

Betton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. Research in Microbiology. (153): 399-404.