Difference between revisions of "Part:BBa K339006:Design"
 
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===Design Notes===
 
===Design Notes===
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None
 
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The cpxP promoter was extracted from the <i>E. coli</i> genome through PCR using primers designed by literature (Shimohata et al. 2002). The XbaI restriction site was added in front of the gene and the full Biobrick suffix (SpeI, NotI, and PstI cut sites) were added to the end.
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===Source===
 
===Source===
  
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Amplified from the ''E. Coli'' Genome using primers designed from literature data.
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The primer sequences used to isolate <i>cpxP</i> from the <i>E. coli</i> genome were 5′-CCGGAATTCTGTTTAAATACCTCCGAGGC (Forward) and 5′-CGCGGATCCGCTCCCAAAATCTTTCTGTCG (Reverse). (Shimohata et al. 2002)
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===References===
 
===References===
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Shimohata, N., Chiba, S., Saikawa, N., Ito, K., and Akiyama, A., 2002., The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with a cytosolic active site. <i>Genes to cells</i> 7(7):653-662
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Latest revision as of 22:52, 30 October 2010

CpxP Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cpxP promoter was extracted from the E. coli genome through PCR using primers designed by literature (Shimohata et al. 2002). The XbaI restriction site was added in front of the gene and the full Biobrick suffix (SpeI, NotI, and PstI cut sites) were added to the end.

Source

The primer sequences used to isolate cpxP from the E. coli genome were 5′-CCGGAATTCTGTTTAAATACCTCCGAGGC (Forward) and 5′-CGCGGATCCGCTCCCAAAATCTTTCTGTCG (Reverse). (Shimohata et al. 2002)

References

Shimohata, N., Chiba, S., Saikawa, N., Ito, K., and Akiyama, A., 2002., The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with a cytosolic active site. Genes to cells 7(7):653-662