Difference between revisions of "Part:BBa K339002"

Revision as of 02:01, 30 October 2010

malEΔSS

This is a form of the maltose binding protien (malE) native to E. Coli, tht has the signal sequence removed.

Usage and Biology

With the signal sequence deleted, this protein is not localized to the periplasm, but rather stays in the cytoplasm. Despite this, it is still able to fold well in the cytoplasm. This protein can therefore be used as a fusion partner for hard to express proteins that stay in the cytoplasm of the cell. This part can also be used to characterize cytoplasmic misfolding promoters.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 360
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 58

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K339002 short</partinfo>
-CpxP promoter
+This is a form of the maltose binding protien (malE) native to E. Coli, tht has the signal sequence removed.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+With the signal sequence deleted, this protein is not localized to the periplasm, but rather stays in the cytoplasm.  Despite this, it is still able to fold well in the cytoplasm.  This protein can therefore be used as a fusion partner for hard to express proteins that stay in the cytoplasm of the cell.  This part can also be used to characterize cytoplasmic  misfolding promoters.
 <!-- -->