Difference between revisions of "Part:BBa K329014"
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===Usage and Biology=== | ===Usage and Biology=== | ||
==Proof that IntI1 integrase works !== | ==Proof that IntI1 integrase works !== | ||
Revision as of 01:20, 30 October 2010
RBS - IntI1
IntI1 is an integron integrase of the class 1 multiresistant integron (MRI). The integron platform consists of an integrase gene (intI) belonging to the tyrosine recombinase family and a primary recombination site, attI. The integrase mediates recombination between the attI site and a target recombination sequence called an attC site (or 59-base element). The attC site is usually found associated with a single open reading frame in a circularized structure termed a gene cassette. Insertion of the gene cassette at the attI site, which is located downstream of a resident promoter, Pc, internal to the intI gene, drives expression of the encoded proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 245