Difference between revisions of "Part:BBa K239000:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K239000===
 
===Applications of BBa_K239000===
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We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.
  
 
===User Reviews===
 
===User Reviews===
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The DegP promoter is dependent of Sigma E (and hence also the growth phase) and can not be induced only by over expression of e.g. CpxR. In comparison, the related Spy promoter (BBa_K239001), which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Experiments by the The UCL_London_2009 team have also showed that the activity of the DegP promoter, compared to the spy promoter, is much more dependent of the growth phase of the bacteria.
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For detailed information please visit
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[http://2009.igem.org/Team:UCL_London/From_the_lab/Expts UCL_London Wet Lab Experiments]
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& [http://2009.igem.org/Team:UCL_London/From_the_lab/Results UCL_London Wet Lab Results]
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The University of Calgary iGEM team tested degP with malE and malE31, proteins known to fold and misfold respecitively in the periplasm of E. Coli.  For more information, please visit our Wiki:
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[http://2010.igem.org/Team:Calgary/Parts/Characterization]

Latest revision as of 01:13, 30 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K239000

We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.

User Reviews

UNIQc8613d91f9a53a40-partinfo-00000000-QINU UNIQc8613d91f9a53a40-partinfo-00000001-QINU


The DegP promoter is dependent of Sigma E (and hence also the growth phase) and can not be induced only by over expression of e.g. CpxR. In comparison, the related Spy promoter (BBa_K239001), which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Experiments by the The UCL_London_2009 team have also showed that the activity of the DegP promoter, compared to the spy promoter, is much more dependent of the growth phase of the bacteria.

For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Expts UCL_London Wet Lab Experiments] & [http://2009.igem.org/Team:UCL_London/From_the_lab/Results UCL_London Wet Lab Results]


The University of Calgary iGEM team tested degP with malE and malE31, proteins known to fold and misfold respecitively in the periplasm of E. Coli. For more information, please visit our Wiki: [http://2010.igem.org/Team:Calgary/Parts/Characterization]