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Date | Name | Thumbnail | Size | Description | Versions |
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14:58, 19 September 2015 | BBa K1804001 PlasmidMap.png (file) | 49 KB | Plasmid map of plac-gfp in pSB1C3 vector (BBa_K1804001). | 1 | |
14:47, 19 September 2015 | BBa K1804001 FluorescenceIntensities.png (file) | 19 KB | GFP is expressed in BL21 transformed with pAC-placgfp and pSB1C3. Data is represented in Mean +/- Standard Deviation of GFP fluorescence intensity (A.U.) for the positive control of BL21 pAC-plac-gfp, BL21 pSB1C3-plac-gfp and the negative control BL21. Th | 1 | |
14:43, 19 September 2015 | BBa K1804001 Descriptives.png (file) | 18 KB | Descriptive statistics for the fluorescence intensities of the bacteria cells used in the experimental duplicates (n=15). Descriptive statistics for the negative control were not included as the fluorescence intensities of the negative control were consta | 1 | |
14:37, 19 September 2015 | BBa K1804001 Fluorescence.png (file) | 885 KB | Representative images of bacterial cultures were taken using a confocal laser scanning microscope at 488 nm, in duplicates A. BL21 pAC-plac-gfp, positive control. B. BL21 pSB1C3-plac-gfp. C. BL21, negative control. | 1 | |
14:12, 19 September 2015 | BBa K1804001 Figure2 PCR.png (file) | 74 KB | PCR of correct clone of pSB1C3-plac-gfp using BioBricks Prefix and BioBricks Suffix primers. Marker used is 100 bp ladder. Lanes 1 to 4 are PCRs of pSB1C3-plac-gfp, PCR-purified Junk-Prefix-plac-gfp-Suffix-Junk (positive control), pAC-plac-gfp (negative c | 1 | |
14:06, 19 September 2015 | BBa K1804001 Figure1.png (file) | 151 KB | Plasmid maps of pAC plasmids with (A) EsaR-EsaI (original plasmid from Addgene #47660) or (B) plac-gfp | 1 |