File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 23:17, 29 June 2021 | PhoA substrates.png (file) |  |
29 KB | Figure 2. General mechanism of phosphate monoester hydrolysis via the catalysis from phoA<sup>[3]</sup>. | 1 |
| 00:39, 30 June 2021 | BBa K3767001 phoA colored (flipped).png (file) | .png) |
238 KB | Figure 1: 3D representation of the alkaline phosphatase. </b> A and B domains shown in red and green respectively. Zinc and Magnesium highlighted in grey and blue respectively | 1 |
| 00:48, 30 June 2021 | 3-24 + 40x phoA.png (file) |  |
333 KB | Figure 4. The final ScFv sequence (blue and green) plus the improved phoA (red) respectively. | 1 |
| 01:09, 30 June 2021 | BLAST Scores ScFv.png (file) |  |
33 KB | Figure 5. Folding confidence model obtained for VH and VL confidence values of 0.89 and 0.99 using ABodyBuilder. | 1 |
| 18:11, 3 July 2021 | BBa K3767004 OspA colored.png (file) |  |
299 KB | Figure 1. Colored model of purified OspA from E.Coli. | 1 |
| 15:47, 11 July 2022 | BBa K4347007--bst.PNG (file) |  |
97 KB | Bst polymerase derived from thermophilic bacterium Geobacillus stearothermophilus used in LAMP modelled on PyMol. | 2 |
| 18:07, 11 July 2022 | BBa K4347007-bst taq.PNG (file) |  |
110 KB | Bst polymerase (green) superimposed with Klentaq fragment (white) from Taq polymerase in Pymol. | 1 |
| 18:33, 11 July 2022 | BBa K4347007-bst KF.PNG (file) |  |
120 KB | st polymerase (green) superimposed with Klenow fragment (white) from DNA polymerase I from E.coli in Pymol. | 1 |
| 14:43, 12 July 2022 | BBa K4347007--seaview.PNG (file) |  |
108 KB | Protien sequence alignment of Bst (6MU5) and Klentaq (6QV4) in Seaview. | 1 |
| 17:41, 12 July 2022 | BBa K4347007 K549.PNG (file) |  |
49 KB | YASARA position scan results for possible point mutations at residue K549. | 1 |
| 17:56, 12 July 2022 | BBa K4347007 Q584.PNG (file) |  |
50 KB | YASARA position scan results for possible point mutations at residue Q584. | 1 |
| 18:14, 12 July 2022 | BBa K4347007 K582.PNG (file) |  |
52 KB | YASARA position scan results for possible point mutations at residue K582. | 1 |
| 20:01, 12 July 2022 | BBa K4347007 bst point mut.PNG (file) |  |
87 KB | Full Bst structure with point mutations (orange) in thumb domain. | 1 |
| 20:05, 12 July 2022 | BBa K4347007 bst point mut close.PNG (file) |  |
123 KB | Close up of point mutations in Bst polymerase thumb domain modelled in Pymol. | 1 |
| 14:13, 18 July 2022 | BBa K4347006 sac7e.PNG (file) |  |
16 KB | DNA binding protien "Sac7e" modelled in Pymol | 1 |
| 20:34, 18 July 2022 | BBa K4347006 sac7e w.PNG (file) |  |
29 KB | DNA binding protien "Sac7e" modelled in Pymol. | 1 |
| 20:59, 18 July 2022 | BBa K43470010 bst.PNG (file) |  |
97 KB | Bst polymerase derived from thermophilic bacterium Geobacillus stearothermophilus used in LAMP modelled on Pymol. | 1 |
| 13:27, 19 July 2022 | Bst2.png (file) |  |
109 KB | 1 | |
| 13:47, 19 July 2022 | BBa K4347010 bst fusion wout point mut.PNG (file) |  |
87 KB | Wildtype Bst polymerase fused with Sac7e | 1 |
| 16:37, 20 July 2022 | BBa K4347010 bst full fusion stability.PNG (file) |  |
4 KB | Protien stability score of fused modified Bst polymerase computed from YASARA. | 1 |
| 16:40, 20 July 2022 | BBa K43470011 bst fusion stability.PNG (file) |  |
4 KB | Bst fusion protien thermal stability computed from YASARA. | 1 |
| 17:05, 20 July 2022 | BBa K4347007 stability point muts.PNG (file) |  |
4 KB | Thermal stability of mutated Bst polymerase computed from YASARA. | 1 |
| 19:39, 20 July 2022 | BBa K4347011 bst full fusion.PNG (file) |  |
97 KB | Fully modified Bst polymerase with fusion protien and point mutations modelled in Pymol. | 1 |
| 20:26, 21 July 2022 | BBa K4347000 LAMP.PNG (file) |  |
491 KB | Basic schematic of LAMP reaction from Wong et, al (2017) | 1 |
| 18:52, 22 July 2022 | BBa K4347000 FIP AND F3.PNG (file) |  |
81 KB | Forward inner and forward backwards primers | 1 |
| 18:59, 22 July 2022 | BBa K4347002 BIP AND B3.PNG (file) |  |
74 KB | BIP and B3 from LAMP reaction. | 1 |
| 19:09, 22 July 2022 | BBa K4347002 FL.PNG (file) |  |
75 KB | Forward Loop primer of LAMP | 1 |
| 19:10, 22 July 2022 | BBa K4347005 BL.PNG (file) |  |
79 KB | Backward loop primer in LAMP | 1 |
| 19:18, 22 July 2022 | BBa K4347000 MEME.PNG (file) |  |
24 KB | Conserved motif (MEME) analysis | 1 |
| 02:32, 2 October 2022 | BBa K4347012 sso7d.PNG (file) |  |
16 KB | DNA-BP Sso7d | 1 |
| 03:10, 2 October 2022 | BBa K4347012 sso7d fusion.PNG (file) |  |
117 KB | DNA-BP Ssso7d fused to modified Bst polymerase | 1 |
| 03:18, 2 October 2022 | BBa K4347011 expression.PNG (file) |  |
227 KB | Expression of sac7e fusion protien | 1 |
| 03:29, 2 October 2022 | BBa K4347012 expression Sso7d.PNG (file) |  |
215 KB | Expression and purification of Sso7d fusion polymerase | 1 |
| 03:33, 2 October 2022 | BBa K4347012 wb.PNG (file) |  |
126 KB | Western blot of purified sso7d polymerase | 1 |
