File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 21:31, 4 December 2016 | GFP Production with Varied Arsenate Concentration.png (file) |  |
91 KB | We transformed our plasmid containing the arsR promoter and arsR gene, amp resistance, and GFP into JM109 E. Coli cells. Wildtype JM109 wild type cells have been reported to have arsenic resistance up to 20 mM (Edmundson, M.C., Horsfall, L. 2015. Front... | 2 |
| 21:45, 4 December 2016 | Plasmid Map.png (file) |  |
105 KB | This plasmid map displays all the elements for the recombinant plasmid to be transformed into E. coli. All elements are contained in the plasmid Coli Comet Cassette-stuffer_Amp-HC (available from DNA 2.0) except for the arsR elements (colored yellow).... | 1 |
| 17:41, 5 December 2016 | Time Course Study.png (file) |  |
334 KB | We grew JM109 E. Coli cells that had been transformed with our plasmid in 0 to 15 mM KH2AsO4 concentrations for 12 hours to determine the time at which the cells began producing GFP. The total culture volume was 250uL with starting OD600 0.01 of cells... | 2 |
| 17:47, 5 December 2016 | Time Course.png (file) |  |
334 KB | We grew JM109 E. Coli cells that had been transformed with our plasmid in 0 to 15 mM KH2AsO4 concentrations for 12 hours to determine the time at which the cells began producing GFP. The total culture volume was 250uL with starting OD600 0.01 of cells... | 1 |
| 17:49, 5 December 2016 | Dose Response.png (file) |  |
91 KB | Because we did not observe maximum GFP production for arsenate concentrations under 15 mM, we performed a dose response study with arsenate concentrations ranging from 0 mM to 30 mM. We grew cells in a total volume of 1.5mL LB in 37°C for 24 hours in... | 1 |
