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Date Name Thumbnail Size Description Versions
16:27, 18 October 2009 GPF, long induction.jpg (file) 26 KB Membrane localization test using fluorescence microscopy. The upper picture shows GFP and the lower picture shows the live cells. (a) is the yeast transformed with pESC-Fusion-GFP induced with galactose for 45mins. (b) is the yeast transformed with pESC-F 3
16:28, 18 October 2009 GPF2.jpg (file) 26 KB Membrane localization test using fluorescence microscopy. The upper picture shows GFP and the lower picture shows the live cells. (a) is the yeast transformed with pESC-Fusion-GFP induced with galactose for 45mins. (b) is the yeast transformed with pESC-F 1
16:38, 18 October 2009 FACS.jpg (file) 66 KB FACS results showing the functional assay of chimeric receptor compared with RI7 receptor. A is the RI7 receptor (1)induced or (2) not induced with the ligand hexanal. B is the chimeric receptor (1)induced or (2) not induced with the ligand diacetyle. 1C 1
22:05, 21 October 2009 Primer design.jpg (file) 13 KB Primer design for the chimeric odorant sensing receptor fusion PCR. 2
22:43, 21 October 2009 Experiment2.PNG (file) 134 KB Rat RI7 receptors have been expressed in "Saccharomyces cerevisiae" and functioned to sense hexanal ligand. 2
23:48, 21 October 2009 FACS new2.jpg (file) 138 KB RI7 receptor (1)induced or (2) not induced with the ligand hexanal.1C and 2C indicate DNA contents. 1
23:50, 21 October 2009 FACS new.jpg (file) 80 KB Fig2. FACS results showing the functional assay of chimeric receptor compared with RI7 receptor. A is the RI7 receptor (1)induced or (2) not induced with the ligand hexanal. B is the chimeric receptor (1)induced or (2) not induced with the ligand diacetyl 2
00:15, 22 October 2009 GFP-all.jpg (file) 441 KB Fig1. Membrane localization test using fluorescence microscopy. The upper picture shows GFP and the lower picture shows the live cells. (1) is the yeast transformed with pESC-Fusion-GFP induced with galactose for 45mins. (2) is the yeast transformed with 1
00:17, 22 October 2009 GFP-all2.jpg (file) 441 KB Fig1. Membrane localization test using fluorescence microscopy. The upper picture shows GFP and the lower picture shows the live cells. (1) is the yeast transformed with pESC-Fusion-GFP induced with galactose for 45mins. (2) is the yeast transformed with 1
00:19, 22 October 2009 GFP-all3.jpg (file) 170 KB Fig1. Membrane localization test using fluorescence microscopy. The upper picture shows GFP and the lower picture shows the live cells. (1) is the yeast transformed with pESC-Fusion-GFP induced with galactose for 45mins. (2) is the yeast transformed with 1