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| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 13:43, 29 September 2018 | SAHS 1 Fig1.png (file) |  |
187 KB | In our circuit of the build process, we have been doing nucleic acid gel electrophoresis to verify. After the loop is complete, sequencing verification. | 2 |
| 13:52, 29 September 2018 | SAHS 1 Fig3.png (file) |  |
307 KB | SAHS is an exogenous protein with a signal peptide in front of it that allows the protein to be secreted outside the membrane. But our site organism is E. coli, because there is a cell wall, so the protein can not be secreted out of the membrane. There... | 2 |
| 13:53, 29 September 2018 | SAHS 1 Fig2.png (file) |  |
90 KB | In our circuit of the build process, we have been doing nucleic acid gel electrophoresis to verify. After the loop is complete, sequencing verification. | 2 |
| 14:55, 29 September 2018 | KaiC 2 .gif (file) |  |
234 KB | [1]Akiyama S. Structural and dynamic aspects of protein clocks: how can they be so slow and stable?[J]. Cellular & Molecular Life Sciences Cmls, 2012, 69(13):2147-2160. | 1 |
| 14:57, 29 September 2018 | KaiC 3 .gif (file) |  |
96 KB | [1]Akiyama S. Structural and dynamic aspects of protein clocks: how can they be so slow and stable?[J]. Cellular & Molecular Life Sciences Cmls, 2012, 69(13):2147-2160. | 1 |
| 15:18, 29 September 2018 | All Kai.gif (file) |  |
57 KB | [1]Shultzaberger R K, Boyd J S, Katsuki T, et al. Single mutations in sasA enable a simpler ΔcikA gene network architecture with equivalent circadian properties.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014... | 1 |
| 15:19, 29 September 2018 | KaiC 4 .gif (file) |  |
951 KB | [1]Swan J A, Golden S, Liwang A, et al. Structure, function, and mechanism of the core circadian clock in cyanobacteria.[J]. Journal of Biological Chemistry, 2018, 293(14):5026-5034. | 1 |
| 15:22, 29 September 2018 | Kai ABC 2.gif (file) |  |
761 KB | [1]Swan J A, Golden S, Liwang A, et al. Structure, function, and mechanism of the core circadian clock in cyanobacteria.[J]. Journal of Biological Chemistry, 2018, 293(14):5026-5034. | 1 |
| 15:23, 29 September 2018 | Kai 2 .gif (file) |  |
273 KB | [1]Swan J A, Golden S, Liwang A, et al. Structure, function, and mechanism of the core circadian clock in cyanobacteria.[J]. Journal of Biological Chemistry, 2018, 293(14):5026-5034. | 1 |
| 15:27, 29 September 2018 | PKaiBC 2 .gif (file) |  |
102 KB | [1]Espinosa J, Boyd J S, Cantos R, et al. Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output[J]. Proceedings of the National Academy of Sciences of the United States of America... | 1 |
| 15:33, 29 September 2018 | KaiA.png (file) |  |
68 KB | https://www.uniprot.org/uniprot/Q79PF6 | 1 |
| 15:35, 29 September 2018 | KaiB.png (file) |  |
110 KB | https://www.uniprot.org/uniprot/Q79PF5 | 1 |
| 15:37, 29 September 2018 | KaiC z.png (file) |  |
216 KB | https://www.uniprot.org/uniprot/Q79PF4 | 1 |
| 15:38, 29 September 2018 | KaiC x.png (file) |  |
242 KB | https://www.uniprot.org/uniprot/Q79PF4 | 1 |
| 15:39, 29 September 2018 | CikA.png (file) |  |
74 KB | https://www.uniprot.org/uniprot/Q9KHI5 | 1 |
| 15:42, 29 September 2018 | SasA.png (file) |  |
44 KB | https://www.uniprot.org/uniprot/Q06904 | 1 |
| 05:44, 30 September 2018 | KaiC 1 .gif (file) |  |
142 KB | [1]Akiyama S. Structural and dynamic aspects of protein clocks: how can they be so slow and stable?[J]. Cellular & Molecular Life Sciences Cmls, 2012, 69(13):2147-2160 | 4 |
| 05:40, 4 October 2018 | KaiA Gel.png (file) |  |
56 KB | 1 | |
| 05:45, 4 October 2018 | KaiB Gel.png (file) |  |
52 KB | 1 | |
| 05:48, 4 October 2018 | KaiC Gel.png (file) |  |
33 KB | 1 | |
| 05:54, 4 October 2018 | SasA Gel.png (file) |  |
87 KB | 1 | |
| 05:56, 4 October 2018 | CikA Gel.png (file) |  |
92 KB | 1 | |
| 06:03, 4 October 2018 | RpaA Gel.png (file) |  |
88 KB | 1 | |
| 06:16, 4 October 2018 | SAHS protein Gel.png (file) |  |
132 KB | 2 | |
| 06:23, 4 October 2018 | CAHS Gel.png (file) |  |
50 KB | 1 | |
| 06:24, 4 October 2018 | CAHS protein Gel.png (file) |  |
118 KB | 1 | |
| 06:31, 4 October 2018 | PKaiBC Gel.png (file) |  |
75 KB | 1 | |
| 10:44, 5 October 2018 | SAHS Gel 1.png (file) |  |
356 KB | 1 | |
| 10:46, 5 October 2018 | SAHS Gel 2.png (file) |  |
242 KB | 1 | |
| 10:49, 5 October 2018 | SAHS pro Gel 1.png (file) |  |
400 KB | 1 | |
| 10:54, 5 October 2018 | CAHS Com pro Gel1.png (file) |  |
258 KB | 1 | |
| 08:04, 6 October 2018 | K2623011.png (file) |  |
194 KB | 1 | |
| 08:06, 6 October 2018 | K2623013.png (file) |  |
248 KB | 1 | |
| 10:05, 7 October 2018 | K2623016.png (file) |  |
417 KB | 1 | |
| 17:48, 16 October 2018 | Kai 1 .gif (file) |  |
109 KB | 2 | |
| 18:04, 16 October 2018 | PKaiBC 1 .gif (file) |  |
79 KB | 2 | |
| 18:29, 16 October 2018 | PKaiBC RpaA .gif (file) |  |
207 KB | 2 | |
| 04:38, 17 October 2018 | GroupA.png (file) |  |
23 KB | 1 | |
| 04:38, 17 October 2018 | GroupB.png (file) |  |
23 KB | 1 | |
| 06:15, 17 October 2018 | Math.png (file) |  |
47 KB | 1 | |
| 06:24, 17 October 2018 | Mathb.png (file) |  |
49 KB | 1 | |
| 06:48, 17 October 2018 | A fitting.txt (file) |  |
2 KB | 1 | |
| 06:49, 17 October 2018 | B fitting.txt (file) | |
2 KB | 1 | |
| 12:43, 17 October 2018 | Figure 9- the circuit of BBa K2623014.png (file) |  |
48 KB | 1 | |
| 12:53, 17 October 2018 | Figure 10- the E.coli DH5а with BBa-K2623014 and IPTG turns red.(D+ is E.coli DH5а with BBa K2623014 and IPTG, D- is E.coli DH5а with BBa K2623014 without IPTG, D0 is E.coli DH5а without BBa K2623014).png (file) | .png) |
448 KB | 1 | |
| 12:57, 17 October 2018 | Figure 5- gradient dilution.png (file) |  |
123 KB | 1 | |
| 13:01, 17 October 2018 | Figure 5- the CFU of BL21.png (file) |  |
22 KB | 1 | |
| 13:09, 17 October 2018 | Figure 7- the colony recovery rate.png (file) |  |
16 KB | 1 | |
| 13:17, 17 October 2018 | Figure 15- the plate of colony for counting.png (file) |  |
1.06 MB | 1 | |
| 13:33, 17 October 2018 | BBa K2623016.jpg (file) |  |
1.75 MB | 1 |
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