View source for Talk:Problems with PCR using VR/VF2

*'''[[User:Rshetty|RS]] 09:10, 27 August 2007 (EDT)''': At what annealing temperature did you do this expt?  Presumably, increasing the annealing temperature during PCR should mitigate this effect.
**'''[[User:SBurke|Sam]] 13:34, 28 August 2007 (EDT):''' I did a gradient PCR to see if increasing the annealing temperature would help. The results of it are [[VR/VF2_Gradient_PCR_Experiment|here]] and are now linked from the first paragraph.