View source for Promoters/Catalog/Constitutive

[[Image:ConstitutivePromoter.png|200px|right]]
==Constitutive ''E. coli'' promoters==
All the promoters in this section are ''E. coli'' promoters that are '''constitutive''' meaning that their activity is dependent on the availability of RNA polymerase holoenzyme, but is not affected by any transcription factors.  If you find any promoters on this page that you know to be regulated by a particular transcription factor, please let us know, or re-categorize the part yourself!
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===Constitutive ''E. coli'' &sigma;<sup>70</sup> promoters===
This section lists promoters that are recognized by [[Help:Promoters/Prokaryotic RNAP#E. coli RNA polymerases|''E. coli'' &sigma;<sup>70</sup> RNAP]].  &sigma;<sup>70</sup> is the major ''E. coli'' sigma factor so there should be RNAP present to transcribe these promoters under most growth conditions (although maximally during exponential growth).<br>
*[[Promoters/Catalog/Anderson|'''Anderson Promoter Collection''']] - a well-characterized collection of ''E. coli'' &sigma;<sup>70</sup> constitutive promoters contributed to the registry by Chris Anderson.
<parttable>promoter_ecoli_constitutive_sigma70</parttable>

===Constitutive ''E. coli'' &sigma;<sup>S</sup> promoters===
This section lists promoters that are recognized by [[Help:Promoters/Prokaryotic RNAP#E. coli RNA polymerases|''E. coli'' &sigma;<sup>S</sup> RNAP]].  &sigma;<sup>S</sup> is the major stationary phase ''E. coli'' sigma factor.  Use these promoters when you want high promoter activity during stationary phase or during starvation.  Since &sigma;<sup>S</sup> promoters have the same consensus promoter sequence as &sigma;<sup>70</sup> promoters, you may find these promoters will be weakly expressed by &sigma;<sup>70</sup> RNAP.<br>

<parttable>promoter_ecoli_constitutive_sigmaS</parttable>

===Constitutive ''E. coli'' &sigma;<sup>32</sup> promoters===
This section lists promoters that are recognized by [[Help:Promoters/Prokaryotic RNAP#E. coli RNA polymerases|''E. coli'' &sigma;<sup>32</sup> RNAP]].  &sigma;<sup>32</sup> is the major heat shock sigma factor in ''E. coli''.  Use these promoters when you want high promoter activity during heat shock.  These promoters are also active under several different shock responses.

<parttable>promoter_ecoli_constitutive_sigma32</parttable>

===Constitutive ''E. coli'' &sigma;<sup>54</sup> promoters===
This section lists promoters that are recognized by [[Help:Promoters/Prokaryotic RNAP#E. coli RNA polymerases|''E. coli'' &sigma;<sup>54</sup> RNAP]].  &sigma;<sup>54</sup> is a minor ''E. coli'' sigma factor that is most highly expressed during nitrogen-limitation.  Use these promoters if you want promoter activity to depend on ammonia or nitrogen levels.  Alternatively, since this is a minor sigma factor that recognizes promoters with a very different sequence to &sigma;<sup>70</sup> promoters, this sigma factor could be repurposed to be be expressed and active during some user-chosen set of environmental conditions, essentially producing a user controllable RNAP.<br>

<parttable>promoter_ecoli_constitutive_sigma54</parttable>

===Constitutive ''B. subtilis'' &sigma;<sup>A</sup> promoters===
This section lists promoters that are recognized by [[Help:Promoters/Prokaryotic RNAP#E. coli RNA polymerases|''B. subtilis'' &sigma;<sup>A</sup> RNAP]].  &sigma;<sup>A</sup> is the major ''B. subtilis'' sigma factor so there should be RNAP present to transcribe these promoters under most growth conditions (although maximally during exponential growth).<br>

<parttable>promoter_subtilis_constitutive_sigmaA</parttable>

===Constitutive ''B. subtilis'' &sigma;<sup>B</sup> promoters===
This section lists promoters that are recognized by [[Help:Promoters/Prokaryotic RNAP#E. coli RNA polymerases|''B. subtilis'' &sigma;<sup>B</sup> RNAP]].  &sigma;<sup>B</sup> is the major stationary phase ''E. coli'' sigma factor.  Use these promoters when you want high promoter activity during stationary phase or during starvation.  <br>

<parttable>promoter_subtilis_constitutive_sigmaB</parttable>

===Constitutive promoters from miscellaneous prokaryotes===
The promoters in this table are prokaryotic promoters that are '''constitutive''' meaning that the activity of these promoters is dependent only on the concentration of the appropriate RNA polymerase and sigma factor.  Constitutive prokaryotic promoters other than those from ''E. coli'' or ''B. subtilis'' are listed here.  The equivalent pages for ''E. coli'' and ''B. subtilis'' are listed [[Promoters/Catalog/Ecoli/Positive|here]] and [[Promoters/Catalog/B. subtilis/Positive|here]].  Note that many [[Help:Promoters/Prokaryotic RNAP|prokaryotic RNA polymerases]] recognize similar promoter sequences so many of these promoter sequences will work in other prokaryotes.
<parttable>promoter_prokaryote_miscellaneous_constitutive</parttable>

===Constitutive promoters from bacteriophage T7===
{{:Promoters/T7/Constitutive/Overview}}

<parttable>bacteriophage_T7_constitutive_promoter</parttable>

===Constitutive promoters from bacteriophage SP6===
All the promoters in this table are recognized by the SP6 RNA Polymerase and are '''constitutive''' meaning that their activity is dependent on the availability of RNA polymerase, but is not affected by any transcription factors.  SP6 promoters are known to have high activity levels and are also highly orthogonal to bacterial promoters since the respective RNA Polymerases are unrelated.

<parttable>promoter_bacteriophage_SP6_constitutive</parttable>

===Constitutive promoters from yeast===
All the promoters in this table are yeast promoters that are '''constitutive''' meaning that their activity is dependent on the availability of RNA polymerase holoenzyme, but is not affected by any transcriptional regulators.

<parttable>yeast_constitutive_promoter</parttable>

===Constitutive promoters from miscellaneous eukaryotes===
All the promoters in the table below are eukaryotic promoters that are '''constitutive''' meaning that their activity is dependent on the availability of RNA polymerase holoenzyme, but is not affected by any transcription factors.

<parttable>promoter_eukaryote_miscellaneous_constitutive</parttable>


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