View source for Part:BBa R0065

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<partinfo>BBa_R0065 short</partinfo>

cI repressor negatively regulates this promoter and LuxR activates its transcription.The      effect of cI is dominant over LuxR. This part is based on the LuxR and cI      repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires      the binding of two cI repressor dimers for maximal repression and contains      two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky      in the sense that 'some' transcription is seen in the absence of both cI and      LuxR.    

   <table width="75%" border="1">     <tr>       <td><strong>LuxI</strong></td>       <td><strong>cI</strong></td>       <td><strong>activity of promoter</strong></td>     </tr>     <tr>       <td>+</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>+</td>       <td>-</td>       <td>maximum</td>     </tr>     <tr>       <td>-</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>-</td>       <td>-</td>       <td>leaky (no quantitative information)</td>     </tr>   </table>   




===Usage and Biology===

Pretty good 'off' in the absence of LuxR/HSL. [jb, 5/24/04]

*very leaky (no LuxR/HSL) at high (100-300) copy number.  can only be slightly induced by LuxR+3OC6HSL.  (jt, ut-austin; 08/11/05)
* Part has since been improved by the [http://2010.igem.org/Team:MIT 2010 MIT iGEM] team in the form of the part [[Part:BBa_K415032]] where the second -10 promoter region has been removed, and the part is thus less leaky.

<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_R0065 SequenceAndFeatures</partinfo>




==Functional Parameters: Austin_UTexas==
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<partinfo>BBa_R0065 parameters</partinfo>
<h3><center>Burden Imposed by this Part:</center></h3>
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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<figcaption><center><b>Burden Value: 1.2 ± 2.8% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the 
<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p> 
<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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