View source for Part:BBa K5348006


<partinfo>BBa_K5348006 short</partinfo>




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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K5348006 SequenceAndFeatures</partinfo>


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    <title>BBa_K5348006 (pL-RBS3)</title>
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    <h2>BBa_K5348006 (pL-RBS3)</h2>

    <h3>Construction Design</h3>
    <p>
        To reduce the leaky expression of the light-inducible induction system (BBa_K3447133, hereafter referred to as the pL), we reduced the intensity of the RBS linked to the target gene in this element by 150-fold (RBS3). The composition of this element is shown below.
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        <img src="https://static.igem.wiki/teams/5348/bba-k5348006/figure-1.jpg" alt="Figure 1. Schematic diagram of the pL-RBS3">
        <div class="caption">Figure 1. Schematic diagram of the pL-RBS3</div>
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    <h3>Engineering Principle</h3>
    <p>
        Under dark conditions, histidine kinase (YF1) phosphorylates FixJ (response regulator of histidine kinase), which activates PFixK2 (the target gene for transcription upon FixJ activation), driving the expression of the cI gene (λ phage repressor), which represses the transcription of its cognate promoter, PR (the cognate promoter of cI), and downstream genes cannot be expressed. Under blue light, the cI gene cannot be expressed, PR can be transcribed normally, and downstream genes can be expressed [1].
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    <h3>Reference</h3>
    <p>[1] H, Mays RL, Hoffman SM, Avalos JL. Optogenetic Control of Microbial Consortia Populations for Chemical Production. ACS Synth Biol. 2021 Aug 20;10(8):2015-2029.</p>

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