View source for Part:BBa K4960040


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<partinfo>BBa_K4960040 short</partinfo>

A system for targeted delivery of target proteins.<br>
===Profile===
Name: pvc1-pvc12、pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD、pvc14-pvc16<br>
Origin: Synthetic<br>
Properties: A system for targeted delivery of target proteins.<br>

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===Usage and Biology===
Mutant Pvc13-E01-DARPin fused with the DARPin E01 structural domain can enhance the binding ability of PVCs to cells, ultimately enabling targeted delivery of fat proteins. Pvc13-E01-DARPin mutant-based constructs of PVCs particles can specifically target cells with EGFR receptors[1]. In subsequent cell experiments, we used Hela and HEK-293T with high EGFR expression to ensure the targeting of PVC.
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===Sequence and Features===
<partinfo>BBa_K4960040 SequenceAndFeatures</partinfo>
Tube:pvc1, pvc5, pvc7<br>
Sheath:pvc2, pvc3, pvc4<br>
Sheath terminator:pvc15<br>
Tail fibres:pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD<br>
Baseplate:pvc9, pvc11, pvc12<br>
Spike:pvc8, pvc10<br>
Assembly method:3A Assembly<br>
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<img src="https://static.igem.wiki/teams/4960/wiki/composite-part/bsal-replace/beautiful-pvc.png" class="figure-img img-fluid rounded"  height="100px"><br>

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'''Figure 1.PVC composition diagram'''
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===Functional Parameters===
<partinfo>BBa_K4960040 parameters</partinfo>
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===References===
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364.