View source for Part:BBa K4642040:Design


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<partinfo>BBa_K4642040 SequenceAndFeatures</partinfo>


===Design Notes===
In order to design probes for miRPA, we used NUPACK’s design functions in its API to find probes which would bind to the miRNAs, but have overhangs which didn’t bind within themselves, to ensure primers could easily anneal to them. We then tested, using NUPACK, the specificity of these probes and found that they had a less than 1% likelihood of binding to the closest human miRNA homologs in the correct shape. This demonstrated the increase in specificity that using miRPA could add to our test.



===Source===

Custom designed in-silico using NUPACK API.

===References===