View source for Part:BBa K4630113


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<partinfo>BBa_K4630113 short</partinfo>

StgRNA-cassette (1+2) is the ligated form of stgRNA-cassette 1 and stgRNA-cassette 2. It construct the multi-level recorder allowing the biological events to trigger recording twice.

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===Usage and Biology===
In the cassette recorder, we integrate the two methods into a single cassette, with unique barcode (fig 1). Once triggered, double strand break (DSB) is introduced into the working plasmid, enabling the intra-plasmid recombination. It is worth noting that the two homologous arms flank the stgRNA, so the entire cassette will be deleted after the recombination, and the next cassette is expected to be brought to the proper expressing place. The inducer triggers over and over, and recorder records it by keeping changing the cassette.
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                 <img style="margin:20px auto 5px auto;" src="https://static.igem.wiki/teams/4630/wiki/parts/bba-k4630100-stgrna-barcode-cassette-1/designpart.svg" width="50%">
                 <p style="color:Gray; padding:0px 30px 10px;">Figure 1. Working pattern of the change of cassette</p>
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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4630113 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K4630113 parameters</partinfo>
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