View source for Part:BBa K325905

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<partinfo>BBa_K325905 short</partinfo>

This part contains two operons. The first consists of the LuxCDEG genes from V.fischeri that have been codon optimised for use in E.coli. These genes produce the substrate for the LuxAB genes encoded by the second reading frame. The LuxAB genes have been derived from Xenorhabdus luminescens. The first operon can be placed under any promoter to achieve inducible or constitutive light output. The second operon is controlled by the strong constitutive pLambda promoter. 

As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K325905 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K325905 parameters</partinfo>
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