View source for Part:BBa K299816

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<h2><partinfo>BBa_K299816 short</partinfo></h2>


<p> <b>Invasin (INV)</b> plays a key role in the initiation of <i>Yersinia enterocolytica</i> and <i>Yersinia pseudotuberculosis</i> infection. Through interaction with a beta1-integrin receptor present on the surface of eucaryotic cell membranes it triggers a signal-transduction pathway leading to internalisation of the whole bacterium in the endocytosis-dependent manner. The strong affinity of Invasin to it’s receptor results in highly selective binding to the target molecule. Mammalian cells depleted of beta1-integrin cannot be infected.</p>

<p><b>Green Fluorescent Protein</b> is a noninvasive fluorescent marker for gene expression, protein localisation and intracellulat protein targeting. Originally from <i>Aequorea victoria.</i></p>

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<img src="https://static.igem.org/mediawiki/2010/5/57/Inv.jpg" width="35%" align="left" style='margin-right:5px'>
<h4>Left: HeLa cells infected with E. coli Top10 harbouring J23102+INV+GFP+TT construct. Thanks to FRET between Hirsch dye and GFP infected cells can be seen as a mildly-glowing areas containing bright spots of GFP inside. More photos at <a href="http://2010.igem.org/Team:Warsaw/Stage3/Gallery">Gallery of Microphotographs</a>.</h4></div></p>
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<p><h3>Authors:</h3>
Cloned by Joanna Leszczyńska.<br>
Created with the use of part <html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299810">BBa K299810</a></html> cloned by Marta Błaszkiewicz.<br>
Work supervised by Michał Lower at all levels.</p>

<p><h3>Construct design</h3>
The part consists of J23102 promoter and B0032 RBS ligated to inv gene from Yersinia pestis (horizontal gene transfer form Yersinia pseudotuberculosis). GFP as marker. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (<html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299813">BBa_K299813</a> and <a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299815">BBa_K299815</a></html>) To find out more about it's background and design <html><a href="http://2010.igem.org/Team:Warsaw/Stage3">click here</a></html>.</p>

<p><h3>Safety</h3>
Bacteria transformed with this part are invasive microorganisms. All safety precautions must be taken when manipulating with transformed strain. It involves obligatory use of laboratory gloves. Work under laminar is strongly advised. All waste should be autoclaved to avoid accidental gene transfer to other bacteria and potential rise of pathogenic organism.</p>
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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K299816 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K299816 parameters</partinfo>
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