View source for Part:BBa K2922028


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<partinfo>BBa_K2922028 short</partinfo>
===Summary===
Abbreviated as Nimm, the molecular weight of Colicin-N immunity protein is 15 kDa protein, and the protein is encoded by gene 
<i>cni</i>. In wild strains of <i>E.coli</i>,the protein is able to protect cells, which harbors the plasmid ColN encoding Colicin-N, against Colicin-N.<ref>[1]https://link.springer.com/article/10.1007/BF00330613</ref>
This protein will prevent <i>E.coli</i> secreting Colicin-N from killing themselves.<ref>[2]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847374/</ref>(Fig.1)
<table><tr><th>[[File:Ndesign.png|thumb|720px|Fig.1 Nimm prevents <i>E.coli</i> BL21 (DE3) secreting Colicin-E1 from killing themselves.]]</th><th></table>
===Identification===
When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing.
After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the <i>Xba</i>I and <i>Pst</i>I to cut the plasmid, then we got the target separate fragment-396bp. (Fig.2)
<table><tr><th>[[File:Eimm Nimm.png|thumb|300px|Fig.2 The result of this plasmid cut with enzyme <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table>

===Sequence and Features===
<partinfo>BBa_K2922028 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2922028 parameters</partinfo>
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===Reference===