View source for Part:BBa K2120414


__NOTOC__
<partinfo>BBa_K2120414 short</partinfo>


'''<Description>'''
<br>This is one of our application circuits.

<br>This circuit contains two devices: inhibitor device and killer device. 
<br>Inhibitor device: We select weak constitutive promoter J23109 and strong RBS B0034 to express inhibitor gene ''cI''. 
<br>Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pR and medium RBS B0032 to control the expression level of toxin protein.
<br>We reversed the direction of inhibitor device, in order to reduce the influence between two devices.
<br>Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])
 

<!-- Add more about the biology of this part here
===Usage and Biology===

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2120414 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K2120414 parameters</partinfo>
<!-- -->