View source for Part:BBa K1956017


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<partinfo>BBa_K1956017 short</partinfo>

Noise in Device measurement 5

==IIT Madras 2016's Characterization==

[[File:Sp_growth.png|400px|right|Specific growth rates (ln) for Devices]]
[[File:Gfpbyrfp_strength.png|400px|right|Relative strength of Devices]]
[[File:Promoterrbs_noise.png|400px|right|Cumulative Intrinsic Noise of Devices]]

== Experimentation ==
<p>This BioBrick was made to understand the role of RBS and Promoter parts in giving rise to intrinsic noise in <i>E. coli</i> DH5alpha. Expression data for GFP and RFP proteins were obtained using flow cytometry (BD FACS Aria III) at 3hr, 6hr, 9hr and 12hr stage of growth along with cells expressing only GFP, only RFP and none. Cumulative intrinsic and extrinsic noise were measured using modified [http://2016.igem.org/Team:IIT-Madras/Model#Noise_in_Devices| Elowitz formula]. OD600 values for specific growth rate estimation were obtained using Spectrophotometer over an interval of an hour for 12 hours. Given specific growth rates are in it's logarithmic values. This BioBrick can be used to characterize noise and strength of complex devices by cloning this device with given device, which produces a different reporter protein. In graphs, we have [https://parts.igem.org/Part:BBa_K1956014 R11-B32], [https://parts.igem.org/Part:BBa_K1956015 R11-B34], [https://parts.igem.org/Part:BBa_K1956017 J14-B3], [https://parts.igem.org/Part:BBa_K1956018 J17-B34], [https://parts.igem.org/Part:BBa_K1956022 R11-B30] and [https://parts.igem.org/Part:BBa_K1956023 R11-B31]. </p>

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K1956017 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K1956017 parameters</partinfo>
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