View source for Part:BBa K079037

__NOTOC__
<partinfo>BBa_K079037 short</partinfo>

These parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079046 TeT Operator Library].
<br><br>
[[Image:TetAffinity.jpg |right|400px|thumbnail|Repression efficiency of Tet Operators Variants expressed as β-galactosidase percentage activity. 100% Being unrepressed ]]
This subset of operator sequences has been chosen since it allows to cover a wide affinity and repression range


[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079036 K079036]:Tet O is the Wild Type operator sequence regulating Tetracycline resistance genes. The natural sequence exhibit a tigth repression given by an high Tet R bindig affinity 
<br>
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079037 K079037]:TetO-4C is a medium affinity operator obtained through a T-C mutation in the fourth nucleotide on both side of the symmetric consensus sequence. Disrupting this interaction the repression efficiency is about '''12''' time lower than the Wt
<br>
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079038 K079038]: TetO-wt/4C5G is a double mutant that heavily compromises the interaction of TetR with the right half of the binding sequence. The resulting repression is quite weak, more than '''40''' times less stringent than in TetO

<br>
The parts can be individually assembled with [https://parts.igem.org/wiki/index.php?title=Part:BBa_J23103 Constitutive Promoter Family] yielding a collection of Tc inducible promoters with different dynamics.
Design rules and effect of multiple Tet Operator sites on repression strength and cooperativity are scarcely represented in the literature. 
Further investigation in this direction are planned

<!-- Add more about the biology of this part here
===Usage and Biology===

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K079037 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K079037 parameters</partinfo>
<!-- -->