View source for IGEM:MIT/2006/Wintergreen odor biosynthetic system gas chromatograms

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'''Verification of methyl salicylate production from cellular metabolites by wintergreen biosynthetic system'''<br>

We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (<partinfo>BBa_J45700</partinfo>).  We analyzed cultures for methyl salicylate production using gas chromatography.  The wintergreen odor biosynthetic system (<partinfo>BBa_J45700</partinfo>) produced some methyl salicylate (A).  The wintergreen odor generator (<partinfo>BBa_J45120</partinfo>) in the absence of either the salicylate generator (<partinfo>BBa_J45320</partinfo>) or exogenous salicylic acid did not produce methyl salicylate (B).  The retention time of the methyl salicylate peak from <partinfo>BBa_J45700</partinfo> is identical to that of the pure methyl salicylate standard (C).  Most ''E. coli'' strains produce indole.]]