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the 95% prediction interval of glucose positive 〈5mmol/L〉urine sample 〈green〉and glucose negative〈0.1mmol/L〉 urine sample〈red〉，the two intervals can be separated after T> 101 mins . To verify the function , by applying the upper limit of the 95% prediction interval of glucose negative urine sample〈red〉as the “diabetic threshold “ , we performed a test to clarify whether our U coli can differentiate diabetes from control . At time T= 101mis with n= 84 ,  our result shows sensitivity= 95% and if we delay the testing time to T=120mins , the sensitivity will be 100%.〈n=84〉, which proved that our Ucoli do Differentiate diabetic urine with normal