View source for Azotobacter vinelandii

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<p>Although Azotobacter Vinelandii is not commonly used as a model of gram-negative bacteria, there are many features that make us introduce the chassis to iGEM. We think that it is very suitable for genetic engineering based on the features below:
<ol>
<li>Anaerobic intracellular environment in aerobic extracellular environment which could use to express oxygen sensitive protein</li>
<li>most parts in registry optimized for E. coli may also function</li>
<li>can use stable genome integration which could transform a larger size of gene at once</li>
<li>Antibiotic such as ampicillin and kanamycin can be used and iGEM vectors such as PSB1A3 can be used</li>
</ol>
</p>

<h3>Nitrogen-repressible T7 Expression System</h3>

For introducing the stable genome integration, we constructs several biobricks.

Moreover, as many promoter of registry cannot be used in Azotobacter Vinelandii, we introduce a novel T7 dependent system using nifH promote. Features about the protein expression system:
<ol>
<li>Nitrogen inducible—repressed by ammonia and other nitrogen source</li>
<li>Predicted to express better than the original T7 expression system</li>
<li>Make T7 promoter usable in Azotobacter Vinelandii, and hence, make a lot more biobricks could be used in Azotobacter Vinelandii.</li>
</ol>
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<p>You can find the parts constructed for stable integration and T7 expression here: <a href="http://2014.igem.org/Team:Hong_Kong-CUHK/home.html?page=link-biobricks" target="_blank">IGEM 2014 CUHK--biobricks</a></p>
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<groupparts>iGEM014 Hong_Kong-CUHK</groupparts>