View source for Part:BBa K5236021


__NOTOC__
<partinfo>BBa_K5236021 short</partinfo>

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins. 

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG. 

RBS: ribosome binding site. 

BhrPETase N191S: This sequence encodes for a mutated BhrPETase N191S and is derived from Escherichia coli.  

pelB:  The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription.

<!-- Add more about the biology of this part here
===Usage and Biology===

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K5236021 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K5236021 parameters</partinfo>
<!-- -->