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  <h3>VEGFR-like</h3>
  <hr>
  <p>
    This year, the USTC iGEM team<a href="https://2024.igem.wiki/ustc/parts">lianjiewenben</a> has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like.
    In this year's project, we selected pBBR1MCS-2 as our plasmid backbone to construct a series of plasmids, which are used to validate the surface display system, VEGFR-like, and the masking peptides targeting VEGFR-like. And we have verified the expression of several of these plasmids in EcN.
  </p>
  
  <table>
    <tr>
        <td><a href=""></a>Name</td>
        <td><a href=""></a>Type</td>
        <td><a href=""></a>Description</td>
        <td><a href=""></a>Designer</td>
        <td><a href=""></a>Length</td>
    </tr>
    <tr>
        <td><a href="/Part:BBa_K5302000">BBa_K5302000</a></td>
        <td><a href="">Coding</a></td>
        <td><a href="">VEGFR1D2</a></td>
        <td><a href="">Jiacan Ma</a></td>
        <td><a href="">303</a></td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302001">BBa_K5302001</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>8IIU</td>
        <td><a href=""></a>Jiacan Ma</td>
        <td><a href=""></a>378</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302002">BBa_K5302002</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>8IJZ</td>
        <td><a href=""></a>Jiacan Ma</td>
        <td><a href=""></a>378</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302003">BBa_K5302003</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>ZVEGF</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>177</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302004">BBa_K5302004</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>Z3C</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>177</td>
    </tr>

 <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302005">BBa_K5302005</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>miniZ</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>102</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302006">BBa_K5302006</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>V114</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>57</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302007">BBa_K5302007</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>V107</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>57</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302008">BBa_K5302008</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>VEGFR-masking#18</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>93</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302009">BBa_K5302009</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>VEGFR-masking#29</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>84</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302010">BBa_K5302010</a></td>
        <td><a href=""></a>Coding</td>
        <td><a href=""></a>VGB</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>96</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302011">BBa_K5302011</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-mCherry</i></td>
        <td><a href=""></a>Jiacan Ma</td>
        <td><a href=""></a>6483</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302012">BBa_K5302012</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-mcherry</i></td>
        <td><a href=""></a>Jiacan Ma</td>
        <td><a href=""></a>6799</td>
    </tr>

  <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302013">BBa_K5302013</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>LppOmpA-mCherry</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>5940</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302014">BBa_K5302014</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>intimin-mCherry</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6957</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302015">BBa_K5302015</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>AIDA-mCherry</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>7269</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302016">BBa_K5302016</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-VEGFR1D2</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>6150</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302017">BBa_K5302017</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-8IIU</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>6225</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302018">BBa_K5302018</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-8IJZ</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>6225</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302019">BBa_K5302019</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-ZVEGF</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>6024</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302020">BBa_K5302020</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-Z3C</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>6024</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302021">BBa_K5302021</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-miniZ</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>5949</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302022">BBa_K5302022</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-V114</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>5903</td>
    </tr>

<tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302023">BBa_K5302023</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-V107</i></td>
        <td><a href=""></a>Xinyuan Shi</td>
        <td><a href=""></a>5903</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302024">BBa_K5302024</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-VEGFR1D2</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6466</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302025">BBa_K5302025</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-8IIU</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6541</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302026">BBa_K5302026</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-8IJZ</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6541</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302027">BBa_K5302027</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-ZVEGF</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6340</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302028">BBa_K5302028</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-Z3C</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6340</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302029">BBa_K5302029</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-miniZ</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6265</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302030">BBa_K5302030</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-V114</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6220</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302031">BBa_K5302031</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-V107</i></td>
        <td><a href=""></a>Bojun Yang</td>
        <td><a href=""></a>6220</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302032">BBa_K5302032</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-VEGFR1D2-l1masking#18</i></td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6144</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302033">BBa_K5302033</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-VEGFR1D2-l2masking#18</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6144</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302034">BBa_K5302034</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-VEGFR1D2-l1masking#29</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6135</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302035">BBa_K5302035</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-VEGFR1D2-l2masking#29</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6135</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302036">BBa_K5302036</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-l1VGB</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6147</td>
    </tr>

<tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302037">BBa_K5302037</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>OmpA-l2VGB</td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6147</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302038">BBa_K5302038</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-VEGFR1D2-l1masking#18</i></td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6496</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302039">BBa_K5302039</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-VEGFR1D2-l2masking#18</i></td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6496</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302040">BBa_K5302040</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-VEGFR1D2-l1masking#29</i></td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6487</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302041">BBa_K5302041</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-VEGFR1D2-l2masking#29</i></td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6487</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302042">BBa_K5302042</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-l1VGB</i></td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6499</td>
    </tr>
    <tr>
        <td><a href="https://parts.igem.org/Part:BBa_K5302043">BBa_K5302043</a></td>
        <td><a href=""></a>Plasmid</td>
        <td><a href=""></a>pBBR-<i>INP-l2VGB</i></td>
        <td><a href=""></a>Dekun Zhou</td>
        <td><a href=""></a>6499</td>
    </tr>
  </table>

  <h3>Reference</h3>
  <hr>
  <p>
    [1]Ivan Guryanov*, Viktor Korzhikov-Vlakh, Madhushree Bhattacharya, Barbara Biondi, Giulia Masiero, Fernando Formaggio, Tatiana Tennikova, Arto Urtt. Conformationally Constrained Peptides with High Affinity to the Vascular Endothelial Growth Factor.Journal of Medicinal Chemistry. Vol 64/Issue 15.July 16, 2021<br>[2]Pan B, Li B, Russell SJ, Tom JY, Cochran AG, Fairbrother WJ. Solution structure of a phage-derived peptide antagonist in complex with vascular endothelial growth factor. J Mol Biol. 2002 Feb 22;316(3):769-87. doi: 10.1006/jmbi.2001.5370<br>[3]Checco JW, Kreitler DF, Thomas NC, Belair DG, Rettko NJ, Murphy WL, Forest KT, Gellman SH. Targeting diverse protein-protein interaction interfaces with α/β-peptides derived from the Z-domain scaffold. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4552-7. doi: 10.1073/pnas.1420380112<br>[4]Rossella Di Stasi, Donatella Diana, Domenica Capasso, Rosanna Palumbo, Alessandra Romanelli, Carlo Pedone, Roberto Fattorusso, Luca D. D'Andrea. VEGFR1D2 in drug discovery: Expression and molecular characterization.19 November 2010<br>[5]Anna Fedorova, Kerry Zobel, Herman S. Gill, Annie Ogasawara, Judith E. Flores, Jeff N. Tinianow, Alexander N. Vanderbilt, Ping Wu, Y. Gloria Meng, Simon-P. Williams, Christian Wiesmann, Jeremy Murray, Jan Marik, Kurt Deshayes. The Development of Peptide-Based Tools for the Analysis of Angiogenesis. Chemistry & Biology. Volume 18, Issue 7. 2011. Pages 839-845. ISSN 1074-5521<br>[6]Balsera B, Bonache MÁ, Reille-Seroussi M, Gagey-Eilstein N, Vidal M, González-Muñiz R, Pérez de Vega MJ. Disrupting VEGF-VEGFR1 Interaction: De Novo Designed Linear Helical Peptides to Mimic the VEGF13-25 Fragment. Molecules. 2017 Oct 28;22(11):1846<br>[7]Lei Wang,Lingyu Zhou,Marie Reille-Seroussi,Nathalie Gagey-Eilstein,Sylvain Broussy, Tianyu Zhang,Lili Ji,Michel Vidal*,Wang-Qing Liu.Identification of Peptidic Antagonists of Vascular Endothelial Growth Factor Receptor 1 by Scanning the Binding Epitopes of Its Ligands.July7.2017<br>[8]Sonia Nicchi1,2, Maria Giuliani1 , Fabiola Giusti1 , Laura Pancotto1 , Domenico Maione1 , Isabel Delany1 , 
    Cesira L. Galeotti1 and Cecilia Brettoni1*.Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of diferent display systems.2021
  </p>
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