View source for Part:BBa K5236020


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<partinfo>BBa_K5236020 short</partinfo>

This composite part codes for the mutated BhrPETase N205G and expresses the enzyme in the extracellular space of chassis. 

T7 promoter: used to regulate gene expression of recombinant proteins. 

Lac operator: used to control gene expression by lactose. 

RBS: the location to bind ribosome. 

BhrPETase N205G: This sequence encodes for a mutated BhrPETase N205G and is derived from Escherichia coli.  

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane. 

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.

<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/n205g.png" width = "50%"><br></html></center>
<center>Fig.1 The result of pET28a-pelB-BhrPETase(N205G)-6xHis DNA sequencing  </center>

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K5236020 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K5236020 parameters</partinfo>
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