View source for Part:BBa K5208003


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AiiM encodes for an N-acyl homoserine lactonase in M. testaceum StLB037. This enzyme catalyzes the hydrolysis of N-Acyl homoserine lactone (AHL) (Wang et al., 2010).

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<figcaption><center>Figure 1. Degradation of N-acyl homoserine lactone (AHL) by AiiM. The dashed line on the left shows where the enzyme breaks the bond (Chen et al., 2013; Dong et al., 2000).</center></figcaption>
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<span class='h3bb'>Sequence and Features</span>
===Sequence and Features===
<partinfo>BBa_K5208003 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K5208003 parameters</partinfo>
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===Usage and Biology===
AiiM degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AiiM in the probiotic Bacillus subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals.

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<figcaption><center>Figure 2. Gel electrophoresis of PCR product of AiiM. </center></figcaption>
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==References==
Chen, F., Gao, Y., Chen, X., Yu, Z., & Li, X. (2013). Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. Int J Mol Sci, 14(9), 17477-17500. https://doi.org/10.3390/ijms140917477 

Coquant, G., Grill, J. P., & Seksik, P. (2020). Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity. Front Immunol, 11, 1827. https://doi.org/10.3389/fimmu.2020.01827 

Dong, Y. H., Xu, J. L., Li, X. Z., & Zhang, L. H. (2000). AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A, 97(7), 3526-3531. https://doi.org/10.1073/pnas.97.7.3526 

Hlordzi, V., Kuebutornye, F. K. A., Afriyie, G., Abarike, E. D., Lu, Y., Chi, S., & Anokyewaa, M. A. (2020). The use of Bacillus species in maintenance of water quality in aquaculture: A review. Aquaculture Reports, 18, 100503. https://doi.org/https://doi.org/10.1016/j.aqrep.2020.100503 

Wang, W. Z., Morohoshi, T., Ikenoya, M., Someya, N., & Ikeda, T. (2010). AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum. Appl Environ Microbiol, 76(8), 2524-2530. https://doi.org/10.1128/AEM.02738-09