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===Usage and Biology===

Spinach 2.1 RNA can bind and activate the fluorophore [http://pubs.acs.org/doi/abs/10.1021/ja410819x DFHBI-1T], both in vivo and in vitro. RNA molecules can thus be fluorescently tagged, by gene fusion with this BioBrick.
The flanking tRNA comes from human tRNALys3 and increases stability and folding efficiency of the RNA, leading to higher fluorescent signal.
It is possible to detect signal from Spinach2.1 when expressed from moderate strength promoters, e.g. the [[Promoters/Catalog/Anderson|Anderson promoter library]].

This part does not contain or need a Ribosome Binding Site.