View source for Part:BBa K4788006


__NOTOC__
<partinfo>BBa_K4788006 short</partinfo>

Transforming cholesterol into 4-cholesten-3-one while producing short chain fatty acids.

<!-- Add more about the biology of this part here-->
===Usage and Biology===
It is derived from the combination of the IsmA gene and the BCoAT gene, which conforms to the part. It can directly degrade cholesterol while producing short chain fatty acids to indirectly regulate cholesterol.
===Characterization===
In order to characterize the corresponding components, we need to develop different characterization schemes for different genes. For the BCoAT gene, We use gas chromatography to examine the production of short chain fatty acids in strains containing the BCoAT gene to characterize the gene, and for the IsmA gene, we used the OPA method to detect cholesterol residue after cholesterol culture medium cultivation to characterize it.
Meanwhile, as short chain fatty acids are also important cholesterol regulatory substances, we can also construct the IsmA-BSH-BCoAT triplet gene to achieve more complex cholesterol regulation. For more information on triplets, please refer to <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4788007"> BBa_K4788007</a ></html>. 
In the characterization of the triad, as shown in Figure 1, we can see that the IsmA gene in the I-BC gene has been successfully characterized. As shown in Figure 2,We can also see the characterization of the BCoAT gene from its GC data.However, the characterization of BCoAT in the binary system is not significant

<html>
<figure style="text-align:center;">
                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4788/wiki/proof/cholesterol.jpg" alt="control">
                <figcaption><b>Figure1:</b> a) Cholesterol residue in the culture medium after cultivation of engineering bacteria on basic cholesterol medium b) Percentage of cholesterol degradation in the culture medium after cultivation of engineering bacteria on basic cholesterol medium</figcaption>
              </figure>
</html>
<html>
<figure style="text-align:center;">
                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4788/wiki/proof/bcoat.jpg" alt="control">
                <figcaption><b>Figure1:</b> GC detection of short chain fatty acid production by BCoAT containing strains</figcaption>
              </figure>
</html>

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4788006 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K4788006 parameters</partinfo>
<!-- -->