View source for Part:BBa K4621151


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<partinfo>BBa_K4621151 short</partinfo>
===Usage, Biology and Characterization===

EctOperon contains ectA, ectB, ectC and ectD and can produce 5-Hydroxyectoine from lysine.

chi18B hypothetical promoter is a functional fragment in chitinase Chi18B gene, which was cloned from SCUT-3 gene. In our project, through transcriptome analysis, qPCR and other means, we found that the gene expression of chitinase Chi18B was up-regulated when SCUT-3 was in chitin medium, and we speculated that its promoter chi18B hypothetical promoter may have properties that can be induced by chitin.

===Testing and validation===;
We ligated the chi18B hypothetical promoter with EctOperon and overexpressed it in Streptomyces SCUT-3 using an integrated plasmid. The modified strain was inoculated in 40 mL LB medium and fermented at 40&#8451; and 220 rpm for 120h. The production of Ectoine and 5-Hydroxyectoin in the presence or without chitin (the first day of fermentation) was detected to evaluate the chitin inducability of the promoter. The results are shown in FIG. 1.
https://static.igem.wiki/teams/4621/wiki/parts/effect-of-different-promoters-on-yield.png

Fig.1 Fermentation of products with or without the addition of an inducer&#65288;chitin) (a).Ectoine (b).5-Hydroxyectoin(72h)



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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4621151 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K4621151 parameters</partinfo>
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