View source for Part:BBa K4779005


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<partinfo>BBa_K4779005 short</partinfo>

UAScit-prm1 is a new synthetic hybrid promoter that improves the basal activity and induced expression level of the prm1 promoter. It consists of two tandem modules, the enhancer element UAScit and the prm1 promoter, where UAScit is a constitutive promoter.
The fluorescence intensity of the engineered yeast strain BY4741-pRS415-UAScit-prm1-GFP-CYC1 was assessed using flow cytometry, and the data were analyzed with FlowJo and GraphPad Prism software. The findings revealed a 13.2% increase in basal activity and a 76.9% increase in induced expression levels compared to the prm1 promoter. This demonstrates that the constitutive enhancer element UAScit enhances both basal and induced expression levels, regardless of whether it is placed in front of inducible or constitutive promoters.


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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4779005 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K4779005 parameters</partinfo>
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