View source for Part:BBa K4165175


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<partinfo>BBa_K4165175 short</partinfo>

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), RBS 4 (BBa_K4165262), 6x His-tag (BBa_K4165020), Tau (BBa_K4165009), and T7 terminator (BBa_K731721).

===Usage and Biology===
In the AD brain, tau Hyperphosphorylation is considered the main cause of AD progression, it may alter the protein's shape and charge, which in turn causes the microtubule-binding domain to become exposed and allow tau to self-assemble and form oligomers characterized to be a neurofibrillary tangle. According to several studies, the polymerized tau (neurofibrillary tangles) is inert since it does not bind to tubulin or encourage its assembly into microtubules, the His Tag for the purification of the protein.

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===<span class='h3bb'>Sequence and Features</span>===
<partinfo>BBa_K4165175 SequenceAndFeatures</partinfo>

===Dry Lab===
<p style=" font-weight: bold; font-size:14px;"> Modeling </p>

<p style=" font-weight: bold; font-size:14px;"> Mathematical modeling </p>
<p style=" font-weight: bold; font-size:14px;">Transcription rate and translation rate under T7 promotor </p>
the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 

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<p><img src="https://static.igem.wiki/teams/4165/wiki/dry-lab/mathematical-modeling/mathematical-modeling/his-tau2.png" style="margin-left:200px;" alt="" width="500" /></p>
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                  Figure 1. this figure shows the results from the transcription and translation code showing 
                 the variation of mRNA and protein concentrations with time compared with the wet lab results.


===WetLab Results===
<p style=" font-weight: bold; font-size:14px;"> Comparison between the mathematical model of dry lab and the wet lab results of His Tau using chemical lysis </p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/tir-wet-expression/his-tau-chem.jpg" style="margin-left:200px;" alt="" width="500" /></p>
</html>
Figure 2. This graph shows the correlation between the mathematical model of dry lab and the wet lab results of His Tau using chemical lysis

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===Functional Parameters===
<partinfo>BBa_K4165175 parameters</partinfo>
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