View source for Part:BBa K4165109


__NOTOC__
<partinfo>BBa_K4165109 short</partinfo>

Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

===Usage and Biology===
Decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with ΔG of 10.3 ± 0.2 kcal/mol.

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===<span class='h3bb'>Sequence and Features</span>===
<partinfo>BBa_K4165109 SequenceAndFeatures</partinfo>

===Dry Lab Characterization===
<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

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table, th, td {
  border:1px solid black; margin-left:auto;margin-right:auto;
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<table style="width:65%">
<table>
  <tr>
    <th>cbeta_deviations</th>
    <th>clashscore</th>
    <th>molprobity</th>
    <th>ramachandran_favored</th>
    <th>ramachandran_outliers</th>
    <th>Qmean_4</th>
    <th>Qmean_6</th>
  </tr>
  <tr>
    <td>0</td>
    <td>17.86</td>
    <td>4</td>
    <td>62.5</td>
    <td>12.5</td>
    <td>-6.45183</td>
    <td>-5.513969</td>
  </tr>
</table>
</body>
</html>


<html>
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1o.jpg" style="margin-left:200px;" alt="" width="500" /></p>
</html>


                        Figure 1.: 3D Model of H1O peptide Modelled by AlphaFold2.0 displayed in Pymol.

===References===
Rey, J., Breiden, M., Lux, V., Bluemke, A., Steindel, M., Ripkens, K., ... & Ehrmann, M. (2022). An allosteric HTRA1-calpain 2 complex with restricted activation profile. Proceedings of the National Academy of Sciences, 119(14), e2113520119. 

<partinfo>BBa_K4165109 parameters</partinfo>
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