View source for Part:BBa K4165059


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<partinfo>BBa_K4165059 short</partinfo>

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), H1A (BBa_K4165000), GS Linker (BBa_K4165017), seed peptide (BBa_K4165012), GS Linker (BBa_K4165019), seed peptide (BBa_K4165012), GS Linker (BBa_K4165017), WAP inhibitor (BBa_K4165008), and T7 terminator (BBa_K731721).



===Usage and Biology===
This part performs a multifunction role in our system, the Plug-Sink system. The H1A (BBa_K4165000) peptide directs the whole peptide to bind to the PDZ HtrA1 protein (BBa_K4165004). The second part is the binding peptide which directs the whole peptide-HtrA1 complex to the tau (BBa_K4165009) and amyloid (BBa_K4165005) aggregates. The last part makes the whole system switch on or off based on the presence of the target protein, thus part is the WAP inhibitor (BBa_K4165008).

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4165051 SequenceAndFeatures</partinfo>



===Dry-Lab characterization===


===Modelling===
PDB Structure:



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<p><img src="https://static.igem.wiki/teams/4165/wiki/39.png" style="margin-left:200px;" alt="" width="500" /></p>
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                    Figure 1. The 3D structure of switch 39 modelled by TRrosetta.